Zeiss ZS-Series User Manual download pdf (Page 14)

G. Image Optimization

G. Image Optimization

G-2. Adjust Channel Settings

1) Make sure the

LSM

2) Select the

SCAN

1)

Make

sure

the

LSM

and ACQUIRE buttons

are selected on the main

toolbar.

2)

Select

the

SCAN

button on the main

toolbar.

4) Use

Pinholes

to

3

)

Select CHANNELS.

4)

Use

Pinholes

to

set pinhole size

(typically, 1-1.5 Airy

units).

)

Use Channel Settings

to adjust settings for

each channel.

5) Use Excitation to set

transmission % for the

laser lines you need.

NOTE

:

As

a

first

NOTE

:

As

a

first

approximation when setting

excitation, the Ar lines should

be set to ~5%. Setting 100%

543 and 20% 633 will result in

1

mW

of

excitation

power

.

Additional wavelengths

can be selected under

LASERLINE.

2/27/2008

14

1

mW

of

excitation

power

.

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