Zeiss Axiovert 135M Specifications Page 1

Axiovert 25 / 25 C / 25 CFLInverted MicroscopeOperating Manual



Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 IX   The Axiovert 25 microscopes and their original acce

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć11 Description1.1 Designation, purposeManufacturer's designation: Axiovert 25 i

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć2Major instrument features are:• Modular design for the optimum performance of

.!&,(*.!&,(*.!&,(*1 11.2.1 Mechanical designAxiovert 25• *+(!% % !%*(* -! (%

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć41.2.2 Optical design(shown using the example of the Axiovert 25 CFL)The timeĆ

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć51.3 Technical data(1) Dimensions and weightDimensions (width x depth x height)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć6Line voltage 100 ... 240 V AC ±10 %. . . . . . . . . . . . . . .

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć7Objectives ICS line of objectives with. . . . . . . . . . . . . . .

Carl Zeiss Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40-012 e 08/99Knowledge of this manual is required for the operation of the instrument. Would

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć81.4 Overview of components1.4.1 Microscope configurationsMicroscope configurations,

* #)%'* #)%'* #)%'- -Microscope configurations, transmitted and reĆflected lightConfigur

LDĆAchrostigmat20x/0,30 Ph1LDĆAchrostigmat40x/0,55 Ph2Figure 1Ć3/1  

Figure 1Ć3/2   Axiovert 25 configurationsAxiovert 25 C microscope stand

#" #" #" $$Figure 1Ć3/3   Axiovert 25 configurationsStepĆdown transformer

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć131.4.2 Accessories for the microscope configurationsAccessories for microscope c

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć14Accessories for microscope configurations ConfigurationOrder No.491207 9804491206

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć15Accessories for microscope configurations ConfigurationOrder No.491207 9804491206

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Format reticule MC 10x/d = 26in the diaphragm part, screwĆin 

1%)/!+-1%)/!+-1%)/!+-3! TABLE OF CONTENTS#!%-&! *#!)*2+%#$-&! )" )(-!(-,    

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e1Ć181.5 Function elements(see Figures 1Ć5, 1Ć6 and 1Ć7 following the table)ItemNo

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 1Ć19ItemNo.Purposeā/ādescriptionDesignation18b Varā/āHā/āVar slider Slider for VAREL c

Figure 1Ć5 Function elements of the Axiovert 2513456789101112131415161718a232527

 Figure 1Ć6 Function elements of the Axiovert 25 C323313456789101112131415161718

Figure 1Ć7 Function elements of the Axiovert 25 CFL3233124567891011121314151617

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć12 Operation2.1 Assembly of the instrument2.1.1 UnpackingThe various models of th

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć22.1.2 Installation(1) Preparations• Place the microscope stand on a suitablework

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć3(3) Insertion of ring diaphragm sliders• Remove the dust protection slider (2

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć4(5) Attachment of the specimen guide M• Attach the specimen guide M for Axi

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć5(6) Connection to the lineIMPORTANT Check whether the voltage indicated on th

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć6(7) Inserting the eyepieces• Remove the dust covers (2Ć8/1) and insert thefix

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć7(9) Using the binocular tube• The eyepiece distance is matched to the indiĆv

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć8(11) āPositioning/switching on the instrument• Unless already done, switch on

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć92.3 Use of LD objectivesWhen inverted microscopes are used for analyses, the

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć102.4 Illumination and contrasting techniquesThe description/application of illumi

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć11Height adjustment of the condenser 0.55The height of the condenser 0.55 can

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć122.4.3 Phase contrast• Preparations as described in section  2.4 and2.4.1.NOTE

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć13NOTE Should the setting range of the centering screws not be sufficient, pl

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć142.4.4 VAREL contrastVAREL contrast provides a reliefĆlike image of specimens

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć15NOTE • Shifting the VAREL illumination to outside the pupil corresponds to o

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć162.4.5 Reflected light fluorescencerequires the use of the Axiovert 25 CFL mi

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć172.5 Working with the specimenguide M and holding frames• Preparations as desc

Figure 2Ć24 Selection of available holding frames123456

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć192.6 Using the focusing eyepieceMeasuring and counting plates or photo reticle

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć202.7 Working with micromanipulatorsThe Axiovert microscopes are prepared as fol

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć222.8.1 Glass stageThe glass stage (2Ć28ā/ā3) enables convenientworking with sim

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 2Ć232.9 Documentation2.9.1 PhotomicrographyThe Axiovert 25 C and 25 CFL microscopes

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e2Ć24• Switch on the microscope; when using color film (artificial light), set t

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 3Ć13 Maintenance and troubleshooting3.1 MaintenanceMaintenance of the the Axiovert

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e3Ć23.2 Troubleshooting and serviceTroubleshooting on the Axiovert 25 is limited t

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 3Ć3(2) Change of halogen lampProceed as follows to change the lamp:• Disconnect

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e3Ć4(3) Change of HBO 50 lampThe HBO 50 illuminator is used for reflected ligh

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 3Ć5Safety notes for useCAUTION The HBO 50 is under high pressure during operat

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e3Ć6• Use the SW 3 screwdriver to loosen the clamping screw on the heatsink an

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 3Ć7Lamp adjustmentCAUTION Never look directly into theignited lamp in order to

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 e3Ć8(4) Change of reflectors FlThe reflectors are equipped in the factory with

 A Change of fluorescence reflectorB Change of fluorescence filter1523491078111

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Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 AĆ3List of key wordsPageAAccessories 1Ć13. . . . . . . . . . . . . . . .

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 eAĆ4PageFilter sets 3Ć8. . . . . . . . . . . . . . . . . . . . . .

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 AĆ5PageMain assemblies 1Ć3. . . . . . . . . . . . . . . . . . . . .

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL08/99 B 40Ć012 eAĆ6PageService 3Ć2, 3Ć10. . . . . . . . . . . . . . . . . . . . . .

Axiovert 25, Axiovert 25 C, Axiovert 25 CFLB 40Ć012 e 08/99 AĆ7  BP BandpassBr. Spectacle wearerC CameraCB Conversion BlueCorr

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/!'-)+/!'-)+/!'-)+4•   !,)* !&+)+ !& +  +/+ ) &,%)

 GENERAL VIEWAxiovert 25 CFL