Zeiss Axiovert 200 User's Guide Page 1

1 MIU User Guide For Zeiss AxioVert 200 Inverted Fluorescence Microscope Molecular Imaging Unit (MIU) University of Helsinki www.miu.helsink

10 As a last step, adjust the iris diaphragm using the slider above the DIC condenser (Fig. 5). First, fully open it and then, if needed, close it as

11 Focusing is done by using the focusing knobs on either side of the microscope. The refresh rate of live image can be adjusted (Fig. 14). Quality o

12 Figure 13 Camera settings in AxioVision software In the image window there is a "French flag" button in the tool bar at the b

13 The recommended minimum camera resolutions for each objective are: Objective NA Recommended min. resolution 10x 0.3 3900 x 3090 20x 0.4 2600

14 BestFit shows the whole range excluding specified percentage of actual minimum and actual maximum values Min/Max shows the whole range from actual

15 When you click with the right mouse button and select VIEW ANNOTATIONS, you will remove the scale bar. If you select VIEW ANNOTATIONS again, the s

16 You can save images temporarily to that folder and later move them into your own computer. The folder will be emptied occasionally (it may happen

17 taken!). There is a 3 GB limit for each user. It is recommended to transfer images somewhere else after your imaging session.

18 5. Ending your imaging session When you have finished your imaging session you should: - Close the fluorescence light shutter (Fig. 3) - Log off

19 The whole image is too bright: Is the transmitted light (halogen bulb) off (p. 5)? Cannot see the whole field of view: Is the fluorescence filter

2 CONTENTS 1. General ... 3 1.1 Instrum

20 6.3 Computer It’s not possible to log on to the computer: Use HYAD user name. Check that the domain selected is ATKK. I do not have HYAD-username:

3 1. General 1.1 Instrument This user guide covers the use of the Zeiss AxioVert 200 inverted epifluorescence microscope. For more information on eac

4 1.5 Users’ mailing list All registered users are automatically added to the users’ mailing list. Please read those e-mails to know what is going on

5 Figure 1 Adjustment of the light path to a correct port Arrow 1: ocular/camera selection Arrow 2: selection of the front/side port. Red circle:

6 2.2 Objectives There are following objectives in the microscope: Position Type Magn. NA Oil/Dry Phase 1 Plan-Neofluar 10x 0.3 Dry Ph1 2

7 2.4 Transmitted (bright-field) light microscopy Transmitted light microscopy is used for specimens that are stained, or have natural pigment so the

8 - Select a 10x objective and DIC .3-.4 condenser position by rotating manually the condenser wheel - Focus on the specimen - Close luminous

9 Pull the analyzer located on the left side of the microscope to the left so that it is in position shown in (Fig. 10). Figure 7 The polarizer fo