Zeiss ZS-Series User Manual download pdf (Page 11)

Zen always collects a Z-stack from

the bottom to the top

Note:

-- Total Z-series collection time is: "Scan time" for one focus position multiplied by the

number of "slices" (focus points). 30-45 minutes collection times are common.

-- To best match the manual live focus position always fopcus from coverslip side to

slide side (i.e. positive to negative). If you go past go back down and approach

again.

-- Optimal section step is half the Airy section thick. This is to satisfy the the Nyquist

sampling criteria

-- Transmitted images are NOT confocal. Do not collect T-PMT in Z-series.

Z-SERIES CAPTURE

1. Find and focus your sample and set capture

parameters as normal.

2. Select Z-STACK in configuration setup area.

3. Open focus tools. SHOW ALL selected. Click ZERO

POSITION ö MANUALLY. to reset current focus position

as "Zero" position.

4. Confirm clicked.

1-AU

5. Use Live view. Focus past the top of your sample,

come back to the top, and Stop live. [If using sequential

scanning, you only need one channel to set focus]

6. Open Z-STACK TOOLS. Check Position if the position

is a positive number click "Set First".

[Positive is towards the slide, negative to towards the

coverslip]

7. Focus to the bottom of your sample. In Z-STACK

TOOLS: Check Position if the position number is

negative. click "Set Last".

8. Click the < OPTIMAL> setting button to set interval (stepsize)

9. Check "brightness" of the middle of your sample (the brightest region), make sure you will not

over-saturate when collecting the images here.

10. Click "Start Experiment" to collect the Z-series

Zeiss 710 Confocal – v1.22 01/29/2015 Page 11

1 2 ... 6 7 8 9 10 11 12

No comments

Zeiss ZS-Series User Manual Page 11