Zeiss AxioCam MRc5 User Manual download pdf (Page 3)

strip" under Measure in main menu — red arrow). This will give the Live — AxioCamMRc5 "live

image box" that will have the image (red circle). You can also click the Workarea box (green

arrow) to show the workarea window (green circle).

Remember to pull OUT both silver metal "sticks" (below the

camera, on right side) for light to the camera from the objective

lens; push bottom stick IN for viewing through microscope ocular

/ eyepiece.

At the bottom left of the Live — AxioCamMRc5 box (circled in

blue) is the Objective box for whatever objective lens is being

used (for the correct micrometer scaling; e.g.. shown below is for

10x objective).

To view the overall image, View in the main menu has Zoom tool

options, and one can start with the Auto Zoom feature (shown

below). Auto Zoom has the advantage of easily displaying all of

the field of view within a reasonable-sized Live image window

box. The (+) Zoom and (-) Zoom features allow one to left click on

the image to quickly increase or decrease the image size. Right

click on image also has menu containing zooms etc.

Selecting Ruler will show the micrometer scale on the image frame at the x (upper) and y (left)

axis (see pg. 8).

For camera speed, "Slow" is preferred for stationary, fixed samples; the slower speed gives

better quality / higher resolution

images.

To get the working AxioCamMRc5 box (to the right of the large Live — AxioCamMRc5 image

box), click on the Properties icon (right-most icon at the bottom of the Live — AxioCamMRc5

image box — highlighted in light blue); when Adjust tab is selected from the menu, the

exposure time, white balance, and image histogram (pixel intensity / RGB scale) functions are

presented (see below, pg. 5). There are icons at the bottom of the Live — AxioCamMRc5 image

box for various scaling features related to brightness / contrast / image gamma (i.e., best fit,

min/max, linear, gamma 0.45).

Best Fit is: optimum of the minimum and maximum contained gray or color value excluding

specified minimum/maximum values.

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Zeiss AxioCam MRc5 User Manual Page 3